Composite

Part:BBa_J70310

Designed by: Tom Knight   Group: Knight Lab   (2009-06-16)


Mesoplasma florum pBG7AU plasmid origin

This is a biobricked version of the circular plasmid pBG7AU, Genbank NC_002569. The part is cut at the end of the Orf1 coding region, following the transcriptional terminator. This is similar to the location of the cut site used in the pKMK1 construction of Dybvig et al (King94) which formed their plasmid p2D4.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 99
    Illegal NheI site found at 120
    Illegal NheI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/mflorum
Parameters
None